Coding

Part:BBa_K2560042:Design

Designed by: Tobias Hensel   Group: iGEM18_Marburg   (2018-09-18)


Phytobrick version of sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


Design Notes

This part was created by polymerase chain reaction (PCR) on the template BBa_BBa_K2560002 and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.

Forward oligo: AACGTCTCGCTCGAATGCGTAAAGGCGAAGAGCTG

Reverse Oligo: TTCGTCTCCCTCAAAGCTTTGTACAGTTCATCCATACCATGC

Source

As Source DNA we used PYTK from the Dueber Toolbox. (Lee et al. 2015.)